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1. The purity of a solute collected between two times t1 and t2 during chromatographic separation can be calculated as
(A): amount of solute eluted - amount of impurity eluted
(B): amount of solute eluted / amount of impurity eluted
(C): amount of solvent eluted + amount of impurity eluted
(D): amount of solvent eluted / amount of impurity eluted
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